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KMID : 0385219970070010031
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Korean Journal of Gerontology
1997 Volume.7 No. 1 p.31 ~ p.35
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Age-Dependent Changes of Langerhans Cells in the Skin of Senescence-Accelerated Mouse (SAM)
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Lee Hyoung-Woo
Chun Inn-Ki
Kim Young-Pil
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Abstract
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Aging is a chronic degenerative process showing the morphological and functional changes in cells and organs. Among these changes, it is well known that the number of Langerhans cells, which are responsible for cellular immunity in the skin, is decreased with aging. SAM was reported to be an useful animal in aging study, but the changes in Langerhans cells with aging in the skin of the strain have not been reported so far. As for the evaluation of eligibility of the SAM in studying the cutaneous aging, the changes in the number and morphology of Langerhans cells with aging were evaluated in the SAM/P8 strain, and ICR strain as a normal control. The two strains were divided into 5 different age-groups; 3, 4, 7, 8, 11 and 14-month-old, and each group comprised 5 animals. Identification of Langerhances cells was performed by ATPase staining method in the epidermis separated from the dermis using EDTA solution. The number and morphology of the cells were counted and observed by a light microscopy. The number of Langerhans cells was significantly decreased with aging in both strains. In the same age groups of both strains, SAM/P8 showed lower than ICR in number of Langerhans cells (p<0.01) except 7-month-old groups (p=0.03). The morphological changes showing loss of dendritic processes of the cells were noticed in 11-month-old and 14-month-old age groups of SAM/P8, which was not observed in all age-groups of ICR strain. These findings suggest that SAM/P8 could be an useful animal model for studying cutaneous aging, especially of immunological aspect.
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KEYWORD
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Langerhans cell, Cellular immunity, Skin aging, Senescence-accelerated mouse(SAM), ICR Strain, Dermis, Epidermis
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